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  • Title: [Purification and properties of intercellular inorganic pyrophosphatase from Saccharomyces cerevisiae].
    Author: Gou P, Yang S.
    Journal: Wei Sheng Wu Xue Bao; 1998 Jun; 38(3):229-32. PubMed ID: 12549338.
    Abstract:
    An inorganic pyrophosphatase (EC3.6.1.1) from Saccharomyces cerevisiae was purified to PAGE homogeneity by sonication disruption, (NH4)2SO4 fractionation and DEAE-cellulose column chromatography. The optimum pH and temperature of the enzyme were 7.4-7.8 and 60 degrees C, respectively. The Km was 19.3 mmol/L. The enzyme required Mg2+ as a cofactor for hydrolysis of pyrophosphate and was inhibited by Ca2+, Hg2+, Pb2+, Mn2+.
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