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  • Title: Immunoreactive gastrin in differential and density gradient fractions of rat gastric mucosa.
    Author: Trotman CN, Fiddes IJ, Grund ER, McHanwell S, Sanders DJ, Sanderson C, Shaw B.
    Journal: J Endocrinol; 1976 Jan; 68(1):5-12. PubMed ID: 1255065.
    Abstract:
    Immunoreactive gastrin was measured in subcellular fractions of rat gastric mucosa. The sedimentational properties of subcellular gastrin-containing structures were distinct from those of mitochondria. After centrifugation in sucrose density gradients using a zonal rotor, the peak of immunoreactive gastrin was found in 1-17--1-18 g cm(-3) density sucrose (1-35 M; 39-5%, W/W). A thermolabile component with 125I-labelled gastrin-binding activity present in gastric mucosal homogenates and fractions was not associated with the gastrin storage vesicles sedimenting in density gradients.
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