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  • Title: [Expression of xylose isomerase gene(xylA) in Saccharomyces cerevisiae from Clostridium thermohydrosulfuricum].
    Author: Bao X, Gao D, Wang Z.
    Journal: Wei Sheng Wu Xue Bao; 1999 Feb; 39(1):49-54. PubMed ID: 12555401.
    Abstract:
    The Clostridium thermohydrosulfuricum xylA gene encoding xylose(glucose) isomerase was cloned in the yeast expression vector pMA91 under the control of the PGK promoter, resulting in pBX-1, and transformed into Saccharomyces cerevisiae. Production of recombinant xylose isomerase was seen in the a Coomassie stained SDS-PAGE gel and the molecular mass was estimated to be 43 kD. The recombinant xylose isomerase showed the highest activity at 85 degrees C and pH7. The specific activity under these condition was 1.0 U/mg protein. At 30 degrees C and 40 degrees C, the relative activity was reduced to 3.7% and 11%, respectively, of the maximum.
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