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  • Title: [Construction of thermotolerant ethanol-producing yeast by protoplast fusion].
    Author: Wen T, Zhao X.
    Journal: Wei Sheng Wu Xue Bao; 1999 Apr; 39(2):141-7. PubMed ID: 12555419.
    Abstract:
    Protoplast fusion between Saccharomyces cerevisiae A001 and Kluyveromyces Y034 has been studied. A001 is a high alcohol yield yeast and can ferment maltose into ethanol, but can not grow at 45 degrees C. However, Y034 can grow above 45 degrees C but has a lower alcohol yield and can not ferment maltose. We obtained the fusants which were tested in several aspects such as cell morphology, physiological and biochemical feature, isozyme patterns analysis, alcohol fermentation at high temperature(45 degrees C) and so on. The fusant AY023 with the final ethanol concentration of 7.4% has been selected, which is thermotolerant yeast with the highest alcohol yield at 45 degrees C until now.
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