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  • Title: [The effects of reactive oxidants on Bacillus thuringiensis parasporal crystals].
    Author: Wang W, Qian C, Shen J, Yang S.
    Journal: Wei Sheng Wu Xue Bao; 1999 Oct; 39(5):469-74. PubMed ID: 12555530.
    Abstract:
    The effects of hydrogen peroxides(H2O2) and hydroxyl radicals(.OH) on the purified parasporal crystals from Bacillus thuringiensis var. Kurstaki HD-1 were studied by SDS-PAGE and bioassay. The results showed that to some extent H2O2 and .OH produced a direct and deleterious effect. Hydroxyl radicals from the system Fe(II)-EDTA-H2O2 indicated more serious damage than hydrogen peroxides. There was a 44.5% loss in activity against 12-h-old larvae of Heliothis armigera when parasporal crystals were treated with 0.3% H2O2 only. However, 85.2% activity lost when parasporal crystals were treated with 0.3% H2O2 in the presence of iron(II) and EDTA.
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