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  • Title: [Studies on preparation of L-phenylalanine from phenylpyruvic acid by E. coli EP8-10].
    Author: Xu H, Ouyang P, Zhou W.
    Journal: Wei Sheng Wu Xue Bao; 1999 Jun; 39(3):272-4. PubMed ID: 12555547.
    Abstract:
    E. coli EP8-10 was selected from the soil. It was able to produce the transaminase with high activity when it was cultivated on the medium containing peptone and beef extract. Optimum conditions of enzyme reaction was: phenylpyruvic acid's concentration of 0.3-0.5 mol/L, L-Asptaric acid used as amino donor, pH 8.5 37 degrees C. When phenylpyruvic acid was 0.3 mol/L, 48 g/L L-phenylalanine was produced after 6 h with 97% conversion rate.
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