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  • Title: Expression and secretion of the heterodimeric protein interleukin-12 in plant cell suspension culture.
    Author: Kwon TH, Seo JE, Kim J, Lee JH, Jang YS, Yang MS.
    Journal: Biotechnol Bioeng; 2003 Mar 30; 81(7):870-5. PubMed ID: 12557321.
    Abstract:
    It has been suggested that plant cell culture is the most suitable system for producing small-to-medium quantities of specialized, expensive, and high-purity proteins. Here, we report that a heterodimeric protein, human interleukin-12 (hIL-12), was expressed and secreted into culture medium in a biologically active form. A transgenic plant expressing hIL-12 was constructed by sexual crossing of plants that expressed each subunit of the protein. From a piece of transgenic plant, callus was induced and cell suspension culture was established. The biological activity and amount of hIL-12 secreted into culture medium were analyzed using bioassays and ELISA. Analysis of cellular localization demonstrated that the protein was secreted into the culture medium together with its intrinsic signal peptide.
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