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  • Title: Factors involved in concanavalin A agglutination of KB and NC37 cells grown in suspension culture.
    Author: Altenburg B, Steiner S.
    Journal: J Natl Cancer Inst; 1976 Jan; 56(1):197-200. PubMed ID: 1255748.
    Abstract:
    NC37 and KB cells grown in suspension culture remained agglutinable at 23 degrees C by concanavalin A (Con A) after sufficient glutaraldehyde fixation to prevent lateral mobility (clustering) of Con A binding sites. In contrast, at 4 degrees C agglutination of the fixed cells was blocked. No significant differences in 3H-Con A binding were observed between unfixed cells and glutaraldehyde-fixed cells at 23 degrees C and unfixed cells at 4 degrees C. Con A bound to fixed cells at 4 degrees C produced agglutination when the cells were washed and then warmed to 23 degrees C. Thus a cold-sensitive factor unrelated to the binding of Con A or the clustering of Con A sites is necessary for agglutination to occur.
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