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  • Title: [Molecular cloning and amino acid composition analysis of a halophilic thiolase gene].
    Author: Liu T, Zhou P.
    Journal: Wei Sheng Wu Xue Bao; 2002 Aug; 42(4):406-10. PubMed ID: 12557544.
    Abstract:
    5' and 3' end sequence of acaBl gene as primers, the gene of halophilic thiolase from haloarchae, Halobacterium sp. ZP-6 was cloned and its amino acid composition was calculated. Compared with non-halophilic thiolase, the halophilic thiolase contains more negative charge amino acid, less positive amino acid and less strong hydrophobic amino acid, and use preferably small side-chain amino acid. Those suggest that electrostatic screen, hydrophobic effect and surface tension all contribute to halophilic properties of thiolase.
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