These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Determination of D-amino acid oxidase activity in tumour cells.
    Author: Sasamura T, Matsuda A, Kokuba Y.
    Journal: Ann Clin Biochem; 2002 Nov; 39(Pt 6):595-8. PubMed ID: 12564842.
    Abstract:
    BACKGROUND: We evaluated the assay for determining D-amino acid oxidase (DAAO) activity in tumour cells, rat liver and rat kidney for studying the effects of D-amino acid-containing solution on cancer patients. METHODS AND RESULTS: In this method the amount of ammonia produced by the DAAO activity after removal of endogenous ammonia using a Sephadex G25 column was determined. The highest activity was observed in rat kidney, which was almost eight times that found in rat liver. As compared with host tissues, the DAAO activity in tumour cells was considerably less. CONCLUSIONS: This DAAO assay may be useful for analysis of various tissue samples as well as tumour cells.
    [Abstract] [Full Text] [Related] [New Search]