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  • Title: [Circulating antigen detection in schistosomiasis japonica patients by sandwich ELISA using polyclonal and monoclonal antibodies].
    Author: Qiu LS, Feng Z, Zhang YH, Li H.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 2000; 18(2):92-3. PubMed ID: 12567723.
    Abstract:
    OBJECTIVE: To detect circulating antigen in the patients with schistosomiasis. METHODS: Polyclonal antibody(PcAb)-monoclonal antibody(McAb) sandwich ELISA. RESULTS: Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%-91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections. CONCLUSION: PcAb-McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.
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