These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Isolation and expression of tilapia (Oreochromis niloticus) serine 8-type GnRH coding and regulatory sequences.
    Author: Farahmand H, Rahman MA, Sohm F, Hwang GL, Maclean N.
    Journal: Gene; 2003 Jan 30; 304():97-106. PubMed ID: 12568719.
    Abstract:
    The complete Serine 8-type gonadotropin releasing hormone (GnRH) coding sequence with a substantial 5-prime regulatory sequence (5 kb) has been isolated and characterised in Nile Tilapia (Oreochromis niloticus) from a relevant genomic library. The primary structure of the protein precursor was identified for this gene. The promoter efficacy has been tested using 0.6 kb of the GnRH promoter driving a lacZ reporter gene in both cultured spleen cells and transiently expressing zebrafish. In the cell transfection experiments, the average level of beta-galactosidase activity in transfected cells was more than 2.1 (P<0.05) times higher than the control promoter-less vector in five independent cultures indicating that the 0.6 GnRH/lacZ construct is able to express in spleen cells. In addition, the transient expression of the lacZ gene was detected in the brain of G0 zebrafish embryos (Danio rerio) 4 days after fertilisation following egg injection with the construct, which demonstrated the efficacy of the tilapia GnRH promoter.
    [Abstract] [Full Text] [Related] [New Search]