These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Production of HBV surface antigen-specific target cell].
    Author: Zhou Z, Zhang D, Ren H.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 1999 Sep 30; 13(3):231-4. PubMed ID: 12569752.
    Abstract:
    OBJECTIVE: To analyze the hepatitis B virus surface antigen (HBsAg)-specific cytotoxic T-cell (CTL) response during hepatitis development and investigate the effect of some factors on CTL response. METHODS: The recombinant retrovirus plasmid PLXSN-S was tranferred into PA317 by electroporation and the pseudoviruses produced from PA317 were used to infect EL4. Clones which highly expressed HBsAg were selected with limited dilution. RESULTS: A clone expressing-HBsAg the highest (A = 0.85 in supernatant at 48th hour) was obtained which steadly expressed HBsAg in vitro after at least 100 passages. It worked very well when used as target cells in CTL response after C57 mice immunized with PLXSN-S and recombinant adenovirus vector (rAdv-B7-2-S). CONCLUSION: We constructed HBsAg-specific target cell line which steadly expressed HBsAg. It may play an important role in HBsAg CTL response and immunopathogenetic mechanisms of hepatitis B.
    [Abstract] [Full Text] [Related] [New Search]