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  • Title: [Studies on immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum].
    Author: Wen LY, Hotez PJ.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 2001; 19(3):166-8. PubMed ID: 12571945.
    Abstract:
    OBJECTIVE: To analyze the immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum with antigens of various species hookworms at different developmental stages. METHODS: SDS-PAGE and ELIB technique were employed in the study. RESULTS AND CONCLUSION: The protein component of Ac-rAsp-1 was 45 kDa, its immune serum can recognize the antigens of Ac-L3 and Ac-rAsp-1 protein, but not react to the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Nb-A and Ac-rAsp-2 protein. The protein component of Ac-rAsp-2 was 24 kDa, its immune serum can recognize the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Ac-L3 and Ac-rAsp-2 protein, but not react to the antigens of Nb-A and Ac-rAsp-1 protein.
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