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  • Title: PCR assay to detect Bacillus anthracis spores in heat-treated specimens.
    Author: Fasanella A, Losito S, Adone R, Ciuchini F, Trotta T, Altamura SA, Chiocco D, Ippolito G.
    Journal: J Clin Microbiol; 2003 Feb; 41(2):896-9. PubMed ID: 12574311.
    Abstract:
    Recent interest in anthrax is due to its potential use in bioterrorism and as a biowarfare agent against civilian populations. The development of rapid and sensitive techniques to detect anthrax spores in suspicious specimens is the most important aim for public health. With a view to preventing exposure of laboratory workers to viable Bacillus anthracis spores, this study evaluated the suitability of PCR assays for detecting anthrax spores previously inactivated at 121 degrees C for 45 min. The results indicate that heat treatment ensures the complete inactivation of B. anthracis spores without significantly affecting the efficiency of PCR assays.
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