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  • Title: [Antifibrotic effects of genistein and quercetin in vitro].
    Author: Qi LH, Kang LP, Zhang JP, Shi N, Zhang M, Wu TM.
    Journal: Yao Xue Xue Bao; 2001 Sep; 36(9):648-51. PubMed ID: 12580099.
    Abstract:
    AIM: To study the antifibrotic effects of genistein (GE) and quercetin (QU) on rat hepatic stellate HSC-T6 cell proliferation stimulated with platelet-derived growth factor (PDGF), collagen synthesis and type I procollagen messenger RNA (mRNA) expression stimulated with transforming growth factor beta 1 (TGF beta 1). METHODS: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by 3H-proline incorporation assay. Type I procollagen mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: GE (25-70 mumol.L-1) and QU (6.25-50 mumol.L-1) concentration-dependently attenuated PDGF-drive HSC-T6 cell proliferative activity. TGF beta 1-stimulated collagen synthesis was also reduced. This was associated with a decrease of type I procollagen mRNA, indicating an effect at a pretranslational level. CONCLUSION: GE and QU may have therapeutic potential against liver fibrosis by regulating PDGF and TGF beta 1 actions.
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