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  • Title: Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles.
    Author: Dyal A, Loos K, Noto M, Chang SW, Spagnoli C, Shafi KV, Ulman A, Cowman M, Gross RA.
    Journal: J Am Chem Soc; 2003 Feb 19; 125(7):1684-5. PubMed ID: 12580578.
    Abstract:
    We report the stability and enzymatic activity of Candida rugosa Lipase (E.C.3.1.1.3) immobilized on gamma-Fe2O3 magnetic nanoparticles. The immobilization strategies were either reacting the enzyme amine group with a nanoparticle surface acetyl, or amine groups. In the former, the enzyme was attached through a C=N bond, while in the latter it was connected using glutaraldehyde. AFM images show an average particle size of 20 +/- 10 nm after deconvolution. The enzymatic activity of the immobilized lipase was determined by following the ester cleavage of p-nitrophenol butyrate. The covalently immobilized enzyme was stabile and reactive over 30 days.
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