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  • Title: Structure and composition of intracytoplasmic membranes of Ectothiorhodospira mobilis.
    Author: Oyewole SH, Holt SC.
    Journal: Arch Microbiol; 1976 Mar 19; 107(2):167-82. PubMed ID: 1259516.
    Abstract:
    The lamellar membrane stacks of Ectothiorhodospira mobilis were isolated and purified by a combination of lysozyme and osmotic shock treatment, followed by differential and density gradient centrifugation. Preparations of lamellar membranes were enriched at least 2.4-fold in the ratio of bacteriochlorophyll a to protein. Thin-sectioning, negative staining, platinum-carbon shadowing and freeze-etching were used to study the architecture of the membrane units. Both platinum-carbon shadowing and freeze-etching showed the outer-surfaces of the isolated lamellar membrane stacks to be relatively smooth. Particles averaging 7 nm in diameter were seen on several faces following freeze-etching.
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