These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Construction and identification of recombinant shuttle-plasmid with ESAT-6 from Mycobacterium tuberculosis].
    Author: Chen W, Bao L, Xie Y, Hu C, Zhang W, Li X, Zhang H.
    Journal: Hua Xi Yi Ke Da Xue Xue Bao; 2002 Jan; 33(1):35-9. PubMed ID: 12599423.
    Abstract:
    OBJECTIVE: To construct a recombinant BCG secretively expressing ESAT-6 of Mycobacterium tuberculosis. METHODS: alpha-antigen(alpha-Ag) signal sequence and esat-6 gene were amplified from the genome of Bacille Calmette-Guerin (BCG) and Mycobacterium tuberculosis by PCR respectively. esat-6 gene was cloned in E. coli-BCG shuttle-plasmid pMV261 to get pME. Then a new recombinant plasmid pSME was constructed by inserting BCG alpha-Ag signal sequence into pME. RESULTS: The cloned genes alpha-Ag signal sequence and esat-6 were correctly inserted into the vector pMV261, which was confirmed by restriction endonuclease digestion and PCR amplification of pSME. CONCLUSION: pSME was expected to secretively express ESAT-6 of Mycobacterium tuberculosis in BCG. This study provides the possibility of further researches on the development of new anti-tuberculosis vaccine.
    [Abstract] [Full Text] [Related] [New Search]