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  • Title: The application of acetylated Sephadex for the separation of proteolipids.
    Author: Monreal J.
    Journal: Biochim Biophys Acta; 1976 Mar 18; 427(1):15-9. PubMed ID: 1259996.
    Abstract:
    The finding that acetylated Sephadex G 200 swells in chloroform/methanol mixture and still acts as a molecular sieve has permitted for the first time the separation of several proteolipid fractions from brain white matter, two of which appear almost free of phospholipids. This new procedure will be useful not only for a rapid purification of proteolipids and separation of lipids but also for general purposes of protein chemistry in organic media. However, it is predictable that one of the main uses of the method will be in the isolation of membrane carrier and of cholinergic receptor proteins, since these components appear to be of proteolipid(-like) nature.
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