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  • Title: A novel cellulolytic, anaerobic, and thermophilic bacterium, Moorella sp. strain F21.
    Author: Karita S, Nakayama K, Goto M, Sakka K, Kim WJ, Ogawa S.
    Journal: Biosci Biotechnol Biochem; 2003 Jan; 67(1):183-5. PubMed ID: 12619693.
    Abstract:
    A cellulolytic and thermophilic anaerobe was isolated from soil. This bacterium made a halo on a roll-tube culture containing Avicel. Analysis of the PCR-based 16S rRNA gene sequence showed that the bacterium was closely related to Moorella thermoacetica. Scanning electron microscopy showed the bacterium is a rod and has no protuberant structure on the surface of cells growing on cellulose, suggesting that this strain is a non-cellulosomal cellulolytic bacterium. Carboxymethyl cellulase and xylanase activities were detected in the culture broth. A major fermentation product from ball-milled cellulose was acetate. This strain has a potential to convert cellulosic biomass to acetate, directly.
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