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  • Title: Human lymphocyte potassium content during the initiation of phytohemagglutinin-induced mitogenesis.
    Author: Segel GB, Lichtman MA, Hollander MM, Gordon BR, Klemperer MR.
    Journal: J Cell Physiol; 1976 May; 88(1):43-8. PubMed ID: 1262405.
    Abstract:
    The K+ content of human lymphocytes has been examined during the initial 24 hours after exposure of cells to phytohemagglutinin (PHA). We have reconfirmed that lymphocyte K+ exchanges rapidly for extracellular counterions during preparative washing if cells are exposed to PHA. By using a technique to measure cation content which does not require removal of cells from their culture medium, we have shown that K+ does not change for 24 hours following PHA treatment. Previous reports have demonstrated that an enhanced uptake of K+ occurs in lymphocytes treated with PHA. This increased uptake may be a compensatory change for an increased exodus, explaining the failure of K+ to change following lectin treatment.
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