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  • Title: Chromatography of biopolymers and their fragments on ion-exchange derivatives of the hydrophilic macroporous synthetic gel spheron.
    Author: Mikes O, Strop P, Zbrozek J, Coupel J.
    Journal: J Chromatogr; 1976 Apr 28; 119():339-54. PubMed ID: 1262418.
    Abstract:
    New ion-exchange materials have been developed from the synthetic glycol methacrylate gel Spheron: carboxymethyl-Spheron (weakly acidic); phosphonyl-Spheron (medium acidic); sulphonyl-Spheron (strongly acidic); diethylaminoethyl-Spheron (Type 1, weakly basic; Type 2, medium to strongly basic). Information is presented on the chromatographic characteristics of these new, macroporous, hydrophilic and rigid ion-exchangers as determined by experiments on the separation of mixtures of proteins, peptides, amino acids, nucleic acids, oligonucleotides, and nucleotides. The possibility is discussed of the use of ion-exchangers of this type in high-performance liquid chromatography of biopolymers.
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