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  • Title: In-vitro cultivation: a sensitive method for detecting Blastocystis hominis.
    Author: Leelayoova S, Taamasri P, Rangsin R, Naaglor T, Thathaisong U, Mungthin M.
    Journal: Ann Trop Med Parasitol; 2002 Dec; 96(8):803-7. PubMed ID: 12625935.
    Abstract:
    Currently, the detection of human infection with Blastocystis hominis is usually based on the examination under a light microscope of faecal samples, either directly, as 'simple smears', or after some form of concentration. Whether short-term, in-vitro cultivation would increase the sensitivity of such detection remains a matter of controversy. Over 900 fresh stool specimens, from soldiers in the Royal Thai Army, were each checked for the parasite using three methods: simple smears; formalin-ethyl-acetate concentration; and cultivation in Jones' medium. Although 334 of the samples were found to be culture-positive, the parasites were only detected in 142 of the simple smears, and faecal concentration led to an even lower sensitivity (64 positive samples). In-vitro cultivation does seem worthwhile in the detection of B. hominis carriage in field studies.
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