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  • Title: Alpha-oxo semicarbazone peptide or oligodeoxynucleotide microarrays.
    Author: Olivier C, Hot D, Huot L, Ollivier N, El-Mahdi O, Gouyette C, Huynh-Dinh T, Gras-Masse H, Lemoine Y, Melnyk O.
    Journal: Bioconjug Chem; 2003; 14(2):430-9. PubMed ID: 12643754.
    Abstract:
    We describe in this paper the preparation and characterization of semicarbazide glass slides and their use for the fabrication of microarrays using site-specific alpha-oxo semicarbazone ligation. The functional density and homogeneity of the semicarbazide glass slides were optimized by analyzing the reactivity of the layer toward a synthetic glyoxylyl fluorescent probe. Oligonucleotide microarrays were prepared by site-specific immobilization of glyoxylyl oligodeoxynucleotides. The slides were directly used in the hybridization assays using fluorescence detection and displayed a significant gain in sensibility as compared to the aldehyde glass slide/amino oligodeoxynucleotide chemistry. Semicarbazide slides were also used for the immobilization of a biotinylated peptide alpha-oxo aldehyde. The peptide microarrays allowed model interaction studies with streptavidin or an anti-biotin antibody.
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