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  • Title: 2P1, a novel male mouse cDNA specifically expressed during meiosis.
    Author: Hammami-Hamza S, Doussau M, Allemand I, Segretain D, Gasc JM, Finaz C.
    Journal: Int J Dev Biol; 2003 Feb; 47(1):71-6. PubMed ID: 12653254.
    Abstract:
    We screened a mouse germinal cell expression library with a probe derived from Sob1, a human testis-specific cDNA, and identified 2P1, a new mouse cDNA. A database search revealed that 2P1 was 91% identical to ORF1 of E3-3, a rat gene probably involved in the regulation of alternative splicing. Sequencing showed that 2P1 has a destabilization motif in its 3'-untranslated region. Northern blotting showed strong gene expression in the testis and weak expression in the epididymis, with no signal detected in other tissues. RT-PCR analysis confirmed testis and epididymis expression. In situ hybridization revealed that 2P1 mRNA was absent in spermatogonia but expressed in spermatocytes. This last result was confirmed by RT-PCR of FACS isolated primary spermatocytes (pachytene stage). Using RT-PCR, purified spermatids were also shown to express 2P1.
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