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  • Title: Demonstration of a Mg-ATPase and a Na,K-ATPase from the arteria carotis communis of the sheep.
    Author: Preiss R, Banaschak H.
    Journal: Acta Biol Med Ger; 1975; 34(1):37-44. PubMed ID: 126627.
    Abstract:
    Besides the Mg-ATPase, a Na,K-ATPase can be demonstrated in different fractions of smooth muscles of the A. carotis communis of the sheep. The highest activity of Mg-ATPase is observed in the heavy microsomal fraction. The Ca-ion may act as a complete substitute for the Mg-ion in the Mg-ATPase. The proportion of Na,K-ATPase is between 10 and 40%, depending on the preparative conditions used in the individual fractions. Fractionated salt treatment (LiBr, KC1, KBr) improved the assay of Na,K-ATPase but increased strength of the Tris-HC1-buffer considerably reduced its activity.
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