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  • Title: Hybridization-based unquenching of DNA hairpins on au surfaces: prototypical "molecular beacon" biosensors.
    Author: Du H, Disney MD, Miller BL, Krauss TD.
    Journal: J Am Chem Soc; 2003 Apr 09; 125(14):4012-3. PubMed ID: 12670198.
    Abstract:
    There is a keen interest in developing techniques for rapid genetic analysis that do not require labeling of an analyte. Here we demonstrate that fluorophore-tagged DNA hairpins attached to gold films can function as immobilized "molecular beacons". Two DNA hairpins incorporating portions of the Staphlococcus aureus FemA and mecR methicillin-resistance genes were attached to a gold substrate. Upon exposure to the complement, a approximately 26-fold increase in fluorescence intensity was measured corresponding to a 96 +/- 5% quenching efficiency. Studies with nonspecific DNA indicate that DNA hairpins immobilized on a gold surface retain their ability to bind complementary DNA sequences selectively.
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