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  • Title: Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry.
    Author: Bauters TG, Swinne D, Stove V, Nelis HJ.
    Journal: J Clin Microbiol; 2003 Apr; 41(4):1736-7. PubMed ID: 12682172.
    Abstract:
    A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.
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