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  • Title: On-line identification of unstable catalpol derivatives from Jamesbrittenia fodina by LC-mS and LC-NMR.
    Author: Cogne AL, Queiroz EF, Wolfender JL, Marston A, Mavi S, Hostettmann K.
    Journal: Phytochem Anal; 2003; 14(2):67-73. PubMed ID: 12693629.
    Abstract:
    LC-UV-MS analysis of the methanol extract of Jamesbrittenia fodina O. M. Hilliard (Scrophulariaceae) revealed the presence of cinnamic ester derivatives. Two isomeric pairs of these constituents were detected, but could not be isolated. In order to identify these unstable compounds, LC-1H-NMR spectra were obtained for each individual isomer and standard NMR measurements were performed in-mixture. The spectra clearly demonstrated that the fractions consisted of mixtures of cis and trans cinnamoyl catalpol glycoside esters.
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