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  • Title: High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture.
    Author: Shin YJ, Hong SY, Kwon TH, Jang YS, Yang MS.
    Journal: Biotechnol Bioeng; 2003 Jun 30; 82(7):778-83. PubMed ID: 12701143.
    Abstract:
    Recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) has been previously produced in tobacco cell suspension cultures. However, the amount of hGM-CSF accumulated in the culture medium dropped quickly from its maximum of 150 microg/L at 5 d after incubation. To overcome this problem, we sought an expression system in which heterologous gene expression could be induced at high levels. We selected a rice amylase expression system in which the promoter Ramy3D is induced to express recombinant protein by sucrose starvation. This induction system was found to give good yield of recombinant hGM-CSF in transgenic rice cell suspension culture and protease activity of this culture medium was low compared to that of tobacco culture system.
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