These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Prokaryocytic expression of the shortened hepatitis B surface antigen and antigenic analysis].
    Author: Ling N, Ren H, Peng ML, Xu HM, Qing YL.
    Journal: Zhonghua Gan Zang Bing Za Zhi; 2003 Apr; 11(4):209-11. PubMed ID: 12716516.
    Abstract:
    OBJECTIVE: To study the expression of the shortened hepatitis B surface antigen in prokaryocyte and detect the antigenic characters. METHODS: Firstly, the gene fragments coding the 152 and 124 amino acids of the carboxyl terminus of hepatitis B surface antigen (HBsAg) were amplified by polymerase chain reaction (PCR). Secondly, they were cloned to plasmid pBKS+, and the accuracy of those constructions were confirmed by restriction enzyme digestion and DNA sequencing. Then, they were cloned to prokaryocytic expression vector-plasmid pET32a(+). The recombinant plasmids were transfected into E.coli BL21 and induced to express with IPTG. RESULTS: The recombinant plasmids were successfully constructed. In E.coli BL21, the protein was expressed in a fusion fashion and could be recognized by monoclonal antibody against HBsAg with ELISA and Western blot. CONCLUSION: The shortened HBsAg can be expressed in prokaryocyte.
    [Abstract] [Full Text] [Related] [New Search]