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  • Title: Micellar electrokinetic chromatography estimation of size and composition of procyanidins after thiolysis with cysteine.
    Author: Herrero-Martínez JM, Ràfols C, Rosés M, Bosch E, Lozano C, Torres JL.
    Journal: Electrophoresis; 2003 May; 24(9):1404-10. PubMed ID: 12731026.
    Abstract:
    This paper describes the characterization of procyanidin mixtures by acid depolymerization in the presence of cysteine (thiolysis with cysteine) and micellar electrokinetic chromatography (MEKC). Reversed-phase liquid chromatography (RP-HPLC) and MEKC were investigated for the separation of the major components of the depolymerized mixtures (catechins and their cysteinyl derivatives). The solutes could only be effectively separated using MEKC. Two background electrolytes (BGEs) are recommended: (i) 50 mM phosphate at pH 7, containing 40 mM sodium cholate (SC) and 10 mM sodium dodecyl sulfate (SDS); (ii) a BGE with the same composition but containing only 50 mM SDS. The MEKC procedures here reported, are cheap, reliable and fast, and their potential in the determination of the size and composition in procyanidin mixtures has been shown. The proposed MEKC methods were validated by comparison with our intralaboratory reference RP-HPLC method using cysteamine as thiol donor.
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