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  • Title: Measurements of carbon to amide-proton distances by C-H dipolar recoupling with 15N NMR detection.
    Author: Schmidt-Rohr K, Hong M.
    Journal: J Am Chem Soc; 2003 May 14; 125(19):5648-9. PubMed ID: 12733900.
    Abstract:
    A new magic-angle spinning NMR method for measuring internuclear distances between a 13C-labeled site and amide protons is described. The magnetization of the protons evolves under homonuclear decoupling and the recoupled 13C-1H dipolar interaction, which provides simple spin-pair REDOR curves if only one 13C-labeled site is present. The modulation of the amide proton HN is detected via short 1H-15N cross polarization followed by 15N detection. The method is demonstrated on two specifically 13C- and 15N-labeled peptides, with 13C-HN distances from 2.2 to ca. 6 A. This technique promises to be particularly useful for measuring distances between 13C=O and H-15N groups, to identify hydrogen bonds in peptides and proteins.
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