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  • Title: Study of nucleophile binding in the penicillin acylase active center. Kinetic analysis.
    Author: Youshko MI, Bukhanov AL, Svedas VK.
    Journal: Biochemistry (Mosc); 2003 Mar; 68(3):334-8. PubMed ID: 12733976.
    Abstract:
    The influence of the external nucleophile (6-aminopenicillanic acid) on the kinetics of the penicillin acylase-catalyzed acyl transfer reactions was studied using a highly sensitive spectrophotometric assay. An adequate kinetic scheme is suggested based on kinetic analysis of the experimental dependencies of the k(cat) and K(m) values on the nucleophile concentration. The proposed kinetic scheme has been verified by a quantitative description of the above-mentioned experimental dependencies using the set of kinetic parameters obtained from independent experiments. Such an approach can be used for modeling of different penicillin acylase-catalyzed acyl transfer reactions.
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