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  • Title: Metal ion enhancement of fungal growth, gene expression and aflatoxin synthesis in Aspergillus flavus: RT-PCR characterization.
    Author: Cuero R, Ouellet T, Yu J, Mogongwa N.
    Journal: J Appl Microbiol; 2003; 94(6):953-61. PubMed ID: 12752802.
    Abstract:
    AIMS: To determine the effect of mineral ions (e.g., Zn2+, Cu2+, and Fe2+) on the enhancement of fungal growth, total RNA, aflatoxin pathway gene expression, and production of aflatoxin and its precursor O-methylsterigmatocystin (OMST). METHODS AND RESULTS: The influence of the metal ions, as a single or mixed treatments, was observed in submerged cultures of toxigenic Aspergillus flavus through changes in the fungal RNA or aflatoxin pathway gene (omtA) by reverse transcription-polymerase chain reaction (RT-PCR) levels, and also in fungal dry-weight accumulation, aflatoxin and OMST production. CONCLUSIONS: The ion treatments induced changes of fungal total RNA, mRNA levels, associate fungal growth, biosynthesis of aflatoxin and OMST, and enhanced expression of RT-PCR. SIGNIFICANCE AND IMPACT OF STUDY: Demonstrates at the cellular and molecular level, the significant effects of metal ions on both fungal growth and production of aflatoxin.
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