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Title: Purification and characterization of an endochitinase produced by Colletotrichum gloeosporioides. Author: Souza RF, Gomes RC, Coelho RR, Alviano CS, Soares RM. Journal: FEMS Microbiol Lett; 2003 May 16; 222(1):45-50. PubMed ID: 12757945. Abstract: The phytopathogenic fungus Colletotrichum gloeosporioides was analyzed for chitinase activity, the best production occurring at the fourth day. A 43 kDa endochitinase with specific activity of 413 U microg(-1) protein was purified corresponding to a 75% yield. The optima of temperature and pH for the enzyme were 50 degrees C and pH 7.0, respectively. The enzyme showed a high stability at 50 degrees C and pH 7.0. Values of pH from 5.0 up to 7.0 gave, at least, 50% of maximum activity, suggesting a biotechnological application. Further studies are in progress to determine the possible use of this endochitinase in biological control.[Abstract] [Full Text] [Related] [New Search]