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  • Title: [Detection of human papillomavirus gene in biopsies from colon carcinoma by PCR].
    Author: Zhu Q, Cao J, Li S.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 1999 Dec; 13(4):352-4. PubMed ID: 12759975.
    Abstract:
    OBJECTIVE: To detect the human papillomavirus gene (HPVs) in biopsies from colon carcinoma by PCR and to study the relationship between HPV infection and colon carcinoma. METHODS: 46 biopsies from colon carcinoma, 10 biopsies from tissue around carcinoma and 16 control biopsies were collected and DNA were extracted. The HPV DNA in biopsies were amplified by PCR using general primers (GP) and primers for HPV6/11, HPV16/33 and HPV18. RESULTS: 20 out of 46 biopsies from colon carcinoma showed HPV gene positive, HPV6/11, HPV6/33 and HPV18 positivity were 3, 11 and 6 respectively. HPV DNA was found in 1 case from 10 biopsies of surrounding tissue of carcinoma. 16 control biopsies were negative. CONCLUSION: HPV may be related to the etiology of colon carcinoma.
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