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  • Title: Isolation and characterization of a mutant of Neurospora crassa deficient in general amino acid permease activity.
    Author: Rao EY, Rao TK, Debusk AG.
    Journal: Biochim Biophys Acta; 1975 Nov 17; 413(1):45-51. PubMed ID: 127619.
    Abstract:
    A mutant of Neurospora crassa (pm-nbg27) was isolated on the basis of its resistance of p-fluoro-phenylalanine on ammonium-deficient Vogel's medium. This mutant was found to be devoid of both conidial and post-conidial (after 180 min of preincubation) transport activity of all amino acids. Genetic analysis of pm-nbg27 by crossing it to wild-type (74A) resulted in the predicted segregants exhibiting transport characteristics of pm-n, pm-b, pm-g, pm-nb, pm-ng, pm-bg and parental types. The above observations confirm the postulated general amino acid permease system as well as a single genetic locus control of that activity.
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