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  • Title: A fluorescent high-throughput assay for double-stranded DNA: the RediPlate PicoGreen assay.
    Author: Batchelor R, Hagen D, Johnson I, Beechem J.
    Journal: Comb Chem High Throughput Screen; 2003 Jun; 6(4):287-91. PubMed ID: 12769671.
    Abstract:
    The fluorescent PicoGreen reagent for detection and quantitation of double-stranded DNA has been adapted for high-throughput screening: the RediPlate PicoGreen double-stranded DNA assay format. In the RediPlate PicoGreen assay format, the PicoGreen reagent is predistributed and co-dried into either 96- or 384-well microplates with the excipient trehalose. The user resuspends the dried reagents upon adding DNA, and measures the resulting fluorescence after a five minute incubation. Replicate fluorescence measurements on nominally identical wells have less than a 5% coefficient of variation. The assay is linear from 5 to 500 ng/ml DNA in a 200 micro l volume. The RediPlate PicoGreen assay format retains the advantages of the original PicoGreen reagent - sensitivity, speed, and specificity - but in a high-throughput format.
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