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  • Title: Sequences affecting the regulation of solvent production in Clostridium acetobutylicum.
    Author: Scotcher MC, Huang KX, Harrison ML, Rudolph FB, Bennett GN.
    Journal: J Ind Microbiol Biotechnol; 2003 Jul; 30(7):414-20. PubMed ID: 12774196.
    Abstract:
    The high solvent phenotype of Clostridium acetobutylicum mutants B and H was complemented by the introduction of a plasmid that contains either an intact or partially-deleted copy of solR, restoring acetone and butanol production to wild-type levels. This demonstrates that the solR open reading frame on pSOLThi is not required to restore solvent levels. The promoter region upstream of alcohol dehydrogense E (adhE) was examined in efforts to identify sites that play major roles in the control of expression. A series of adhE promoter fragments was constructed and the expression of each in acid- and solvent-phases of growth was analyzed using a chloramphenicol acetyl-transferase reporter system. Our results show that a region beyond the 0A box is needed for full induction of the promoter. Additionally, we show that the presence of sequences around a possible processing site designated S2 may have a negative role in the regulation of adhE expression.
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