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  • Title: [Determination of piceid in commercial Rhizoma Polygoni Cuspidati by HPLC].
    Author: Wang L, Huang L, Zhang M, Wang ZT.
    Journal: Zhongguo Zhong Yao Za Zhi; 2002 May; 27(5):344-7. PubMed ID: 12774322.
    Abstract:
    OBJECTIVE: To improve a RP-HPLC method of determining piceid in commercial Rhizoma Polygoni Cuspidati. METHOD: HPLC method was used, with C18 column (250 mm x 4.6 mm), a mixture of CH3CN-H2O (20:80) as the mobile phase, and detecting wavelength 303 nm. RESULT: The linear range was 0.0066-0.0792 microgram.microL-1, r = 0.9999 (n = 5). The average recovery rate was 98.5% and RSD was 2.5% (n = 9). CONCLUSION: The method is simple, accurate and reproducible. The contents of piceid in commercial Rhizoma Polygoni Cuspidati from different areas are variable and the average content is 2.23%.
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