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  • Title: [Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae].
    Author: Song XJ, Zhang YX, Zhang Y, Zhang QW.
    Journal: Zhongguo Zhong Yao Za Zhi; 2002 Apr; 27(4):267-9. PubMed ID: 12774369.
    Abstract:
    OBJECTIVE: To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae. METHOD: TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (15:85) as mobile phase. The detection wavelength was 327 nm. RESULT: The qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods. CONCLUSION: The methods and data could be used for quality control.
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