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  • Title: [Determination of stilbene derivative in shengfa powders by HPLC].
    Author: Hu BQ, ShI YJ, Huang P.
    Journal: Zhongguo Zhong Yao Za Zhi; 2002 Dec; 27(12):913-5, 948. PubMed ID: 12776530.
    Abstract:
    OBJECTIVE: To develop a new method for the determination of the idiosyncratic component, 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders. METHOD: A HPLC method was set up, using Hypersil BDS C18 column (5 microns, 4.6 mm x 150 mm), acetonitrile-water(18:82) as mobile phase, with detection at 320 nm. RESULT: The calibration curve was linear in the range of 0.45-2.25 micrograms, r = 0.9998, the average recovery was 99.5%, RSD = 1.2%(n = 6). CONCLUSION: The active constituent 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders can be separated effectively. This method is simple, specific and exact.
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