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  • Title: Use of co-immobilized beta-amylase and pullulanase in reduction of saccharification time of starch and increase in maltose yield.
    Author: Atia KS, Ismail SA, El-Arnaouty MB, Dessouki AM.
    Journal: Biotechnol Prog; 2003; 19(3):853-7. PubMed ID: 12790649.
    Abstract:
    Beta-amylase and pullulanase were co-immobilized to poly(acrylamide-acrylic acid) resin [P(AAm-AAc)] using 1-ethyl-3-(3-dimethylaminopropyl) carbodimide hydrochloride (EDC). The combined beta-amylase and pullulanase activity was 32% relative to the nonimmobilized beta-amylase. Co-immobilization of beta-amylase and pullulanase increased the maltose yield compared to thart of the immobilized beta-amylase alone and reduced the saccharification time to about 50 h. The results showed that there is a significant increase in the thermal stability, pH stability, and stability toward gamma irradiation. The results also suggest that the co-immobilization of beta-amylase and pullulanase is a potentially useful approach for commercial starch hydrolysis.
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