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  • Title: Determining acetylcholinesterase inhibitory activity in plant extracts using a fluorimetric flow assay.
    Author: Rhee IK, Appels N, Luijendijk T, Irth H, Verpoorte R.
    Journal: Phytochem Anal; 2003; 14(3):145-9. PubMed ID: 12793460.
    Abstract:
    A fluorometric assay for acetylcholinesterase inhibitory activity was developed in a flow system using the fluorogenic substrate 7-acetoxy-1-methyl quinolinium iodide which is hydrolysed to the highly fluorescent 7-hydroxy-1-methyl quinolinium iodide. The detection limit of galanthamine is 0.5 microM, which is about 20 times more sensitive than in the colorimetric flow assay. In the presence of 30% methanol or of 5% acetonitrile, about 70% of the enzyme activity could still be detected. Various plant extracts have been screened using the described system including bulbs of Galanthus nivalis, Eucharis amazonica (E. x grandiflora), Crinum powelli and Nerine bowdenii (all members of the Amaryllidaceae), which showed strong AchE inhibitory activity.
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