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  • Title: An effective sporicidal reagent against Bacillus subtilis spores.
    Author: Kida N, Mochizuki Y, Taguchi F.
    Journal: Microbiol Immunol; 2003; 47(4):279-83. PubMed ID: 12801065.
    Abstract:
    We developed a reagent which showed significant sporicidal activity against Bacillus subtilis spores. This reagent was composed of ethylenediaminetetraacetic acid, disodium salt (EDTA-2Na), ferric chloride hexahydrate (FeCl3 x 6H2O) and ethanol (tentatively designated as the ethanol reagent). The ethanol reagent showed pH- and temperature-dependent sporicidal activity. At pH 0.3, its activity was almost the same as that of 0.05% sodium hypochlorite at 20 C and was higher at 37 C than at 20 C. The activity of the ethanol reagent was similar both with and without 10% serum. The ethanol reagent might be applicable for disinfecting Bacillus spores.
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