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  • Title: Specific quantification of human genomes from low copy number DNA samples in forensic and ancient DNA studies.
    Author: Alonso A, Martin P, Albarrán C, García P, Primorac D, García O, Fernández de Simón L, García-Hirschfeld J, Sancho M, Fernández-Piqueras J.
    Journal: Croat Med J; 2003 Jun; 44(3):273-80. PubMed ID: 12808718.
    Abstract:
    We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear and mitochondrial DNA regions. Special emphasis has been put on the applicability of recently described different real-time PCR designs targeting different fragments of the HV1 mtDNA control region, and a segment of the X-Y homologous amelogenin gene. The importance of these quantitative assays is to ensure the consistency of low copy number DNA typing (STR profiling and mtDNA sequencing).
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