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  • Title: [In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle].
    Author: Li XD, Hu YY.
    Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi; 2003 May; 17(3):177-9. PubMed ID: 12822343.
    Abstract:
    OBJECTIVE: To determine whether fibroblasts can be used to promote endochondral bone formation in vivo by transfer of human bone morphogenetic protein-2(hBMP-2) into fibroblasts. METHODS: pcDNA3-hBMP-2 was constructed by use of gene clone and recombined technique. NIH3T3 fibroblasts were transfected with pcDNA3-hBMP-2. The positive cell clones were selected with G418. In NIH3T3 fibroblasts transferred with pcDNA3-hBMP-2, the expression of hBMP-2 was determined by in situ hybridization and immunohistochemical analysis; alkaline phosphatase activity was measured. hBMP-2-producing fibroblasts were implanted into nude mouse muscle to observe endochondral bone formation in vivo. RESULTS: pcDNA3-hBMP-2 was successfully constructed. In NIH3T3 fibroblasts transfected with pcDNA3-hBMP-2, the BMP-2 expression was stable; alkaline phophatase activity was much higher than that in non-transfected NIH3T3 cells. Endochondral bone formation in vivo was observed at the site of implantation 4 weeks later. CONCLUSION: Fibroblasts transfected by hBMP-2 gene can be used to promote endochondral bone formation in vivo.
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