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  • Title: Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems.
    Author: Wang J, Oard JH.
    Journal: Plant Cell Rep; 2003 Sep; 22(2):129-34. PubMed ID: 12827439.
    Abstract:
    Strong constitutive promoters form a cornerstone for basic and applied research using transgenic plants. GUS (beta-glucuronidase) expression levels from constructs containing RUBQ1 or RUB2 rice ubiquitin promoters were 8- to 35-fold higher in transgenic rice [Oryza sativa (L.)] plants, respectively, when compared to the 35S promoter. Deletion analysis of the 5'-upstream region of RUBQ2 revealed a putative enhancer region that produced a 2.4-fold increase in transient GUS expression. Southern blot analysis showed that three to seven copies of the GUS gene were stably inserted into R0 and R1 plants and inherited in a monogenic fashion.
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